Effectiveness of the Ruku-ruku Leaf Solution (Ocimum sanctum) as a Natural Preservative in Indian Mackerel (Rastrelliger sp.) during Low-temperature Storage

Main Article Content

Muhammad Heffiqri Riady
Iis Rostini
Yuli Andriani
Rusky Intan Pratama

Abstract

This research was conducted to determine the shelf life of Indian mackerel by giving ruku-ruku leaf solution at different concentrations on phytochemical test, amount of bacteria, degree of acidity (pH), weight loss, and water content in Indian mackerel during low-temperature storage. The study was conducted at the Laboratory of Fisheries Product Processing, University of Padjadjaran, Jatinangor. The research method used is an experimental method with 4 treatments. Ruku-ruku leaf solution treatments concentration were 0%, 10%, 30%, and 50%, soaking time 30 minutes, then stored at low-temperature (5-10ºC). Observations were made on days 1, 3, 6, and 7 for Indian mackerel concentration of 0% or control (without soaking of ruku-ruku leaf solution) while treatment with ruku-ruku leaf solution 10%, 30%, and 50% were carried out at days 1, 3, 6, 7, 8, 9, 10, 11, 12, and 13. The parameters observed included phytochemical test, amount of bacteria, degree of acidity (pH), weight loss, and water content. The results of research showed that the use of a ruku-ruku leaf solution with concentration 30% on Indian mackerel during low-temperature storage has the longest shelf life that is until the 13th day with amount of bacteria 6,90 × 107 cfu/g, degree of acidity (pH ) at 6,95, weight loss at 9,52% and water content at 65,32%.

Keywords:
concentration, Indian mackerel, low-temperature, ruku-ruku leaf solution.

Article Details

How to Cite
Riady, M. H., Rostini, I., Andriani, Y., & Pratama, R. I. (2019). Effectiveness of the Ruku-ruku Leaf Solution (Ocimum sanctum) as a Natural Preservative in Indian Mackerel (Rastrelliger sp.) during Low-temperature Storage. Asian Food Science Journal, 12(3), 1-13. https://doi.org/10.9734/afsj/2019/v12i330084
Section
Original Research Article

References

Heruwati and Endang S. Traditional fish processing: Prospects and development opportunities. Agricultural Research and Development Journal. 2002;21(3).

Puspitasari AF. Identification and prevalence of ectoparasites worms in Mackerel (Rastrelliger sp.) at the nusantara brondong fisheries port, Lamongan. Essay. Faculty of Fisheries and Maritime Affairs. Airlangga University. Surabaya; 2013.

Suryawati A, Meikawati W, Astuti R. Effect of dosage and soaking time for galangal solution on the amount of milkfish bacteria. Indonesian Public Health Journal. 2011;7(1):71-79.

Ilyas S. Fisheries refrigeration technology: Volume I. Fish cooling techniques. CV. Plenary. Jakarta. 1983:237.

Margono. Educational Research Methodology. Rineka Cipta. Jakarta; 2000.

Parnanto NHR, Rini S, Rohula U. Antioxidant capacity and antimicrobial ability in kirinyuh leaves (Eupatorium odoratum) during the storage of Tuna (Euthynnus affinis) at cold temperature. Journal of Agricultural Product Technology. 2013;6(1).

Director general of drug and food control (Ditjen POM). Materia Medika Indonesia. Volume VI. MOH RI. Jakarta. 1989:182-185.

Shafqatullah K. Muhammad, Asadullah, Khakiqurrehman, Ali KF. Comparative analysis of Ocimum sanctum L. stem and leaves for phytochemicals and inorganic constituents. Middle Eats Journal of Scientific Research. 2013;13(2):236-240.

Praveen PK, Ganguly S, Wakchaure R. Ocimum sanctum (Tulsi), the Queen of Herbs: A review. Journal of Biochemistry and Therapeutic Uses of Medical Plants. New Delhi. India; 2017.

Geeta DM. Vasudevan R. Kedlaya S. Deepa, Ballal M. Activity of Ocimum sanctum (The Traditional Indian Medicinal Plant) against the Enteric Pathogens. Indian J. Med. Sci. 2001;55:434-438.

Putranti RI. Skrining fitokimia dan aktivitas antioksidan ekstrak rumput laut Sargassum duplicatum dan Turbinaria ornata dari Japara. Tesis. Fakultas Perikanan dan Ilmu Kelautan. Universitas Diponegoro. Semarang; 2013.

Harborne JB. Phytochemical methods to guide modern ways to analyze plants. 2nd issue. Translators: Padmawinata K. and Soediro I. ITB Publishers. Bandung; 1987.

Fardiaz S. Analysis of Food Microbiology. PT. Raja Grafindo Persada. Jakarta; 1993.

Widiani GD. Use of salam leaf extract to extend the shelf life of red tilapia filet at low-temperature storage. Essay. Faculty of Fisheries and Marine Science. University of Padjadjaran. Jatinangor; 2011.

Afrianto E, Liviawaty E. Handling of fresh fish. Widya Padjadjaran. Bandung; 2010.

Association of Official Analytical and Chemyst (AOAC). Official method of analysis. 18th Ed. Association of Analytical Chemyst, Inc. Maryland, USA; 2007.

Lumowa SVT, Vandalita MMR. Chemical analysis of gamal leaves (Gliricidia sepium) and Pineapple skin (Ananas comosus L) as raw materials for vegetable pesticides. Proceedings of the National Chemistry Seminar; 2017.
[ISBN 978-602-50942-0-0].

Devi S, Tuty M. Antibacterial activity test of ethanol extract of pacar kuku (Lawsonia inermis Linn) Leaves on Pseudomonas aeruginosa Bacteria. Journal of Current Pharmaceutical Sciences. 2017;1(1):31-35.

Dinata A. Overcome dengue larvae with cutaneous skin. Inspiration & P2B2 R&D Ideas Inside. 2008;3(2):59-66.

Cushnie TPT, Lamb AJ. Antimicrobial activity of flavonoids. Int. J. Antimicrob. Agents. 2005;26: 343-356.

Robinson T. High plant organic content. 6th Edition. Translator Prof. Kosasih Padmawinata. ITB. Bandung; 1995.

Santoso MAR, Evi L, Eddy A. Effectiveness of mango leaf extract as a natural preservative for the saving period of tilapia filet at low-temperature. Journal of Fisheries and Maritime Affairs. 2017;8(2):57-67.

Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Review. 1999;12(4): 564-582.

Safitri OM, Nurhamidah, dan Hermansyah A. Potensi sitotoksik dan antibakteri ekstrak daun Laportea interrupta (L.) Chew (Jelatang Ayam) terhadap Staphylococcus aureus. ALOTROP, Journal Pendidikan dan Ilmu Kimia. 2018;2(2):175-183.

Sumarsih S. Mikrobiologi dasar. Universitas pembangunan nasional veteran. Yogyakarta; 2003.

Gelman A, Glatman L, Drabkin V, Harpaz S. Effect of storage temperature and preservative treatment on shelf life of the pond-raised freshwater fish, silver perch (Bidyanus bidyanus). Journal Food Protection. 2001;64:1584-1591.

Dewi EN, Pramitha ND. dan Apri DA. Efektivitas daun ruku-ruku sebagai antibakteri pada ikan kembung lelaki (Rastrelliger kanagurta) selama penyimpanan dingin. Journal Pengolahan dan Bioteknologi Hasil Perikanan. 2015;4(3):1-6.

Sucipto I. Biogas hasil fermentasi hidrolisat bagas menggunakan konsorium bakteri termofilik kotoran sapi. Institut Pertanian Bogor. Bogor; 2009.

Connell JJ. Fish quality control. Fishing News Book, Ltd. London. 1990:222.

Ganiswarna S. Farmakologi dan Terapi. Edisi 4. Penerbit UI. Jakarta; 1995.

Krisanti B. Pengaruh Ekstrak Sargassum sp. terhadap Masa Simpan Filet Ikan Nila Merah pada Suhu Rendah. Skripsi. Fakultas Perikanan dan Ilmu Kelautan. Universitas Padjadjaran. Jatinangor; 2005.

Pelczar Michael J, Chan ECS. Dasar-dasar Mikrobiologi. Jilid II. alih bahasa: Ratna Siri Hadiotomo. UI Press. Jakarta; 1988.

Chaparro-Hernandez S, Ruiz-Cruz S, Marquez-Rios E. Effect of chitosan-carvacrol edible coatings on the quality and shelf life of tilapia (Oreochromis niloticus) Fillets Stored in Ice. Food Sci. Technol, Campinas. 2015;35(4):734-741.

Khalafalla F, Ali F, Hassan A. Quality improvement and shelf-life extension of refrigerated nile tilapia (Oreochromis niloticus) Fillets Using Natural Herbs. Journal of Basic and Applied Sciences. 2015;4(1):33-40.

Fardiaz S. Food Microbiology. Volume I. PT. Gramedia Main Library. Jakarta; 1992.

Damayanti W, Emma R, Zahidah H. Application of chitosan as an antibacterial on patet filet during low-temperature storage. JPHPI. 2016;19(3):321-328.

Afrianto E, Evi L, Otong S, Herman H. Effect of temperature and blanching time on decreasing freshness of tagih filet during storage at low-temperature. Journal of Aquatics. 2014;5(1):45-54.

Junianto. Fish handling techniques. Self-Help Publishers. Jakarta; 2003.

Soeparno. Ilmu dan Teknologi Daging. Gadjah Mada University Press. Yogyakarta; 1994.

Santoso J, Nurjanah Sukarno dan SR. Sinaga. Kemunduran Mutu Ikan Nila Merah (Oreochromis sp.) selama Penyimpanan pada Suhu Chilling. Buletin THP. 1999;1(4).
[ISSN-0854-9230].

Hadiwiyoto S. Fisheries product processing technology. Volume I. Liberty. Yogyakarta; 1993.

Aprianti D. Aktivitas antibakteri ekstrak biji picung (Pangium edule Reinw) dan Pengaruhnya terhadap Stabilitas Fisiko Kimia, Mikrobiologi dan Sensori Ikan Kembung (Rastrelliger neglectus). Skripsi. Fakultas Sains dan Teknologi Universitas Islam Negeri Syarif Hidayatullah. Jakarta; 2011.

Tranggono, Suhardi S. Naruki A. Murdiati, dan sudarmanto. petunjuk praktikum fisiologi dan Teknologi Pasca Panen. PAU Pangan dan Gizi. UGM. Yogyakarta; 1990.

Darsana L, Wartoyo SP, Dan T. Wahyuti. Pengaruh saat panen dan suhu penyimpanan terhadap umur simpan dan kualitas mentimun jepang (Cucumis sativus L.). Agrosains. 2003; 5(1):1-12.

Pomeranz Y. Functional properties of food components. Academic Press, Inc. London; 1985.

Anggraeni DH, Evi L, Rusky IP, Iis R. Effects of concentration of guava leaf extract on the retention period of patin filet based on the amount of microbes. Journal of Fisheries and Maritime Affairs. 2017;8(2):145-151.

Buckle KA, Edwards RA, Fleet GH, Wootton M. Food Science. Purnomo, H. and Adiono translators. University of Indonesia Publisher. Jakarta; 1987:365.

Irianto HE, Soesilo I. Technology support for fisheries product provision. World food day national seminar paper. Maritime and fisheries research agency. Ministry of Maritime Affairs and Fisheries. Bogor; 2007.

Jay JM. Modern food microbiology. Fifth Edition. Chapman and Hall. New York; 1996:490-492.

Adawyah R. Pengolahan dan Pengawetan Ikan. Haka Ghrafis. Jakarta; 2007.

Astawan M. Ikan yang Sedap dan Bergizi. Tiga Serangkai. Solo; 2004.

Kusnandar F. Kimia pangan komponen makro. Dian Rakyat. Jakarta; 2010.

Winarno FG. Food and nutrition chemistry. PT. Gramedia Main Library. Jakarta; 1992:253.

Desniar D. Poernomo dan W. Wijatur. Pengaruh konsentrasi garam pada peda ikan kembung (Rastrelliger sp.) dengan Fermentasi Spontan. Jurnal Pengolahan Hasil Perikanan Indonesia. 2009;12(1):73-87.

Ira. Kajian pengaruh berbagai kadar garam terhadap kandungan asam lemak esensial Omega-3 Ikan Kembung (Rastrelliger kanagurta) Asin Kering. Skripsi. Fakultas Pertanian. Universitas Sebelas Maret. Surakarta; 2008.

Winarno FG. Food and nutrition chemistry. Gramedia Main Library. Jakarta; 2008.

Tapotubun AM, Nanlohy EEEM, Louhenapessy JM. The effect of warming time on the presto quality of some fishes. Ichthyos Journal. 2008;7(2):65-70.

Yanizal Y. Efektivitas air kubis (Brassica oleracea) dalam mengawetkan ikan kembung (Scomber canagorta) di Medan. Fakultas Kesehatan Masyarakat. Universitas Sumatera Utara. Medan; 2010.

Nurjanah Tati N, Asadatun A, dan Ardilla PR. Pengaruh umur panen terhadap komposisi asam lemak ikan gurami (Osphronemus gouramy). Seminar nasional perikanan Indonesia. Departemen Teknologi Hasil Perikanan. Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor. Bogor. 2009:357-358.

Yuarni D, Kadirman dan Jamaluddin. Laju Perubahan Kadar Air, Kadar Protein dan Uji Organoleptik Ikan Lele Asin Menggunakan Alat Pengering Kabinet (Cabinet Dryer) dengan Suhu Terkontrol. Jurnal Pendidikan Teknologi Pertanian. 2015;1:12-21.

National Standardization Agency (BSN). SNI 01-26901-2006. Chemical test methods, Part 2: Determination of water content in fishery products. National Standardization Agency. Jakarta; 2006.

Sari M, Kesuma S. dan Novelina. Pengaruh Penggunaan Daun Kunyit (Curcuma domestica Val.), Daun Ruku-ruku (Ocimum gratissimum L.) dan Daun Mangkokan (Nothopanax cutellarium Merr.) Pada Pengolahan Pindang Presto Ikan Kembung (Rastrelliger sp.) terhadap Mutu Organoleptik dan Daya Awetnya. Prosiding Seminar Perhimpunan Ahli Teknologi Pangan Indonesia. Fakultas Teknologi Pertanian. Universitas Andalas. Padang; 2012.